Biochemical Studies and Characterization of Immobilized Alkaline Phosphatases on Carboxyl-Functionalised Carbon Nanotubes

Authors

  • Vijaya D. Giramkar  Centre for Materials for Electronics Technology, Panchwati, Off Pashan road, Pune, Maharashtra, India
  • Dr. Girish J. Phatak   Centre for Materials for Electronics Technology, Panchwati, Off Pashan road, Pune, Maharashtra, India
  • Prof. Sushma G. Sabharwal  Biochemistry Division, Department of Chemistry, Savitribai Phule Pune University, Pune, Maharashtra, India

Keywords:

Alkaline phosphatase, Immobilization, CNTs, Biochemical studies

Abstract

Alkaline phosphatase has been isolated from the seeds of 'Arachis hypoghaea' by ammonium sulphate fractionation (30-80%) and ion exchange chromatography. The isolated enzyme was studied for its biochemical properties viz. optimum pH and temperature. The purified enzyme was immobilized using carboxyl-functionalised carbon nanotubes (CNTs) by cross linking with epichlorohydrin (ECH) as well as a matrix of ethyl cellulose. The resulting two matrices were characterized by FTIR and SEM to obtain structural and morphological properties. By both methods the immobilised enzyme showed good activity comparable with the native enzyme. Reusability of the immobilised enzyme was demonstrated up to 8-10 cycles. The immobilized enzyme showed higher temperature stability. The investigation of cutting-edge nanomaterial with the help of enzyme immobilisation using exceptional techniques makes nanobiocatalyst of promising thirst for knowledge for biosensor applications.

References

  1. Worsfold, P.J., 1995. Classification and chemical characteristics of immobilized enzyme. Pure Appl. Chem., 67(4): 597-600.
  2. Tu, M., X. Zahang, A. Kurabil, N. Gilkes, W. Mabeel, J. Saddlermaobing, 2006. Immobilization of β-glucosidase on Eupergit C for lignocellulose hydrolysis. Biotechnol. Lett., 28(3): 151-156.
  3. Kim, J., Grate, J. W. Wang, J.P., 2008. Nanobiocatalysis and its potential applications. Trends Biotechnol. 26 (11), 639–646.
  4. Kuchibhatla, S.V.N.T, Karakoti, A. S., Bera, D., Seal, S., 2007. One dimensiona. nanostructured materials. Prog. Mater. Sci. 52 (5), 699–913.
  5. Guo, X.; Zhang, S.; Shan, X. Adsorption of metal ions on lignin. J. Hazard. Mater. 2008, 151, 134–142.
  6. Betancur, M.; Bonelli, P.R.; Velásquez, J.A.; Cukierman, A.L. Potentiality of lignin from the Kraft pulping process for removal of trace nickel from wastewater: Effect of demineralization. Bioresour. Technol. 2009, 100, 1130–1137.
  7. Bulgariu, L.; Bulgariu, D.; Malutan, T.; Macoveanu, M. Adsorption of lead(II) ions from aqueous solution onto lignin. Adsorp. Sci. Technol. 2009, 27, 435–445.
  8. Harmita, H.; Karthikeyan, K.G.; Pan, X.J. Copper and cadmium sorption onto kraft and organosolv lignins. Bioresour. Technol. 2009, 100, 6183–6191.
  9. Ahmaruzzaman, M. Industrial wastes as low-cost potential adsorbents for the treatment of wastewater laden with heavy metals. Adv. Colloid Interface Sci. 2011, 166, 36–59.
  10. Enhanced Film-Forming Properties for Ethyl Cellulose and Starch Acetate Using N-Alkenyl Succinic Anhydrides as Novel Plasticizers. Eur. J. Pharm. Sci. 2003, 19, 363–371.
  11. Dx.doi.org/10.1021/sc400252e | ACS Sustainable Chem. Eng. 2013, 1, 1474−1479

Downloads

Published

2018-01-20

Issue

Section

Research Articles

How to Cite

[1]
Vijaya D. Giramkar, Dr. Girish J. Phatak ,Prof. Sushma G. Sabharwal, " Biochemical Studies and Characterization of Immobilized Alkaline Phosphatases on Carboxyl-Functionalised Carbon Nanotubes, International Journal of Scientific Research in Science and Technology(IJSRST), Online ISSN : 2395-602X, Print ISSN : 2395-6011, Volume 5, Issue 1, pp.197-201 , January-February-2018.