Biochemical Studies and Characterization of Immobilized Alkaline Phosphatases on Carboxyl-Functionalised Carbon Nanotubes

Authors

  • Vijaya D. Giramkar  Centre for Materials for Electronics Technology, Panchwati, Off Pashan road, Pune, Maharashtra, India
  • Dr. Girish J. Phatak   Centre for Materials for Electronics Technology, Panchwati, Off Pashan road, Pune, Maharashtra, India
  • Prof. Sushma G. Sabharwal  Biochemistry Division, Department of Chemistry, Savitribai Phule Pune University, Pune, Maharashtra, India

Keywords:

Alkaline phosphatase, Immobilization, CNTs, Biochemical studies

Abstract

Alkaline phosphatase has been isolated from the seeds of 'Arachis hypoghaea' by ammonium sulphate fractionation (30-80%) and ion exchange chromatography. The isolated enzyme was studied for its biochemical properties viz. optimum pH and temperature. The purified enzyme was immobilized using carboxyl-functionalised carbon nanotubes (CNTs) by cross linking with epichlorohydrin (ECH) as well as a matrix of ethyl cellulose. The resulting two matrices were characterized by FTIR and SEM to obtain structural and morphological properties. By both methods the immobilised enzyme showed good activity comparable with the native enzyme. Reusability of the immobilised enzyme was demonstrated up to 8-10 cycles. The immobilized enzyme showed higher temperature stability. The investigation of cutting-edge nanomaterial with the help of enzyme immobilisation using exceptional techniques makes nanobiocatalyst of promising thirst for knowledge for biosensor applications.

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International Journal of Scientific Research in Science and Technology

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Published

2018-01-20

Issue

Volume 5, Issue 1, January-February-2018

Section

Research Articles

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This work is licensed under a Creative Commons Attribution 4.0 International License.

How to Cite

[1]
Vijaya D. Giramkar, Dr. Girish J. Phatak ,Prof. Sushma G. Sabharwal, " Biochemical Studies and Characterization of Immobilized Alkaline Phosphatases on Carboxyl-Functionalised Carbon Nanotubes, International Journal of Scientific Research in Science and Technology(IJSRST), Online ISSN : 2395-602X, Print ISSN : 2395-6011, Volume 5, Issue 1, pp.197-201 , January-February-2018.